HCID 86593615, compound → Biotin

HRID 1295535Notes

Reaction equation

HRID 1295535 reaction equation
[OH:1][C:2]([CH2:4][CH2:5][CH2:6][CH2:7][C@H:8]1[C@@H:16]2[C@@H:11]([NH:12][C:13]([NH:15]2)=[O:14])[CH2:10][S:9]1)=[O:3].CS(C)=O>O>[OH:3][C:2]([CH2:4][CH2:5][CH2:6][CH2:7][C@H:8]1[C@@H:16]2[C@@H:11]([NH:12][C:13]([NH:15]2)=[O:14])[CH2:10][S:9]1)=[O:1]

Participants

3

Reactant 1

HCID 86593615HCID 86593615, compound

Product 1

HCID 171548Biotin

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

The reagents used for the biotinylation and avidination are the following: 1.24 ml of DMSO was added to 1.1 mg of Biotin-AC5-OSu (manufactured by Dojindo Molecular Technologies, Inc.) to give 1.95 mM of biotin-DMSO solution. 950 l of pure water was added to 50 l of the biotin-DMSO solution, to give 0.0976 mM of biotin solution. The biotin solution thus obtained was designated as liquid A (apparox. 0.1 mM of biotin solution). 0.09 mg of fluorescent dye conjugated avidin (Avidun, Alexa Flour 488 conjugate: manufactured by Molecular Probes, Inc.) was dissolved in 900 L of 0.5M sodium phosphate buffer (pH 7.1), which was designated as liquid B.

Sources & evidence

ORD record
ord-6ff77947405245ad8fced8cb152f92ec
Source dataset
uspto-grants-2007_07
Patent number
US07244499B2

Reaction notes

Loading…