HCID 86593615, compound → Biotin

Participants

Reaction Steps

The reagents used for the biotinylation and avidination are the following: 1.24 ml of DMSO was added to 1.1 mg of Biotin-AC5-OSu (manufactured by Dojindo Molecular Technologies, Inc.) to give 1.95 mM of biotin-DMSO solution. 950 l of pure water was added to 50 l of the biotin-DMSO solution, to give 0.0976 mM of biotin solution. The biotin solution thus obtained was designated as liquid A (apparox. 0.1 mM of biotin solution). 0.09 mg of fluorescent dye conjugated avidin (Avidun, Alexa Flour 488 conjugate: manufactured by Molecular Probes, Inc.) was dissolved in 900 L of 0.5M sodium phosphate buffer (pH 7.1), which was designated as liquid B.

Provenance & Evidence