alpha-Maltotriose → beta-Maltose

HRID 1353993Notes

Reaction equation

HRID 1353993 reaction equation
[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@@H:12]([O:17][C@H:18]3[C@H:23]([OH:24])[C@@H:22]([OH:25])[C@@H:21]([OH:26])[O:20][C@@H:19]3[CH2:27][OH:28])[O:11][C@@H:10]2[CH2:29][OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33].[CH2:35]([OH:79])[C@H:36]1[O:41][C@H:40]([O:42][CH2:43][C@H:44]2[O:49][C@H:48]([O:50][C@H:51]3[C@H:56]([OH:57])[C@@H:55]([OH:58])[C@@H:54]([O:59][C@H:60]4[C@H:65]([OH:66])[C@@H:64]([OH:67])[CH:63]([OH:68])[O:62][C@@H:61]4[CH2:69][OH:70])[O:53][C@@H:52]3[CH2:71][OH:72])[C@H:47]([OH:73])[C@@H:46]([OH:74])[C@@H:45]2[OH:75])[C@H:39]([OH:76])[C@@H:38]([OH:77])[C@@H:37]1[OH:78]>C([O-])(=O)C>[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@H:12]([OH:17])[O:11][C@@H:10]2[CH2:29][OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33].[CH2:35]([OH:79])[C@H:36]1[O:41][C@H:40]([O:42][CH2:43][C@H:44]2[O:49][C@H:48]([O:50][C@H:51]3[C@H:56]([OH:57])[C@@H:55]([OH:58])[C@@H:54]([O:59][C@H:60]4[C@H:65]([OH:66])[C@@H:64]([OH:67])[CH:63]([OH:68])[O:62][C@@H:61]4[CH2:69][OH:70])[O:53][C@@H:52]3[CH2:71][OH:72])[C@H:47]([OH:73])[C@@H:46]([OH:74])[C@@H:45]2[OH:75])[C@H:39]([OH:76])[C@@H:38]([OH:77])[C@@H:37]1[OH:78].[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@@H:12]([O:17][C@H:18]3[C@H:23]([OH:24])[C@@H:22]([OH:25])[C@@H:21]([OH:26])[O:20][C@@H:19]3[CH2:27][OH:28])[O:11][C@@H:10]2[CH2:29][OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]

Participants

6

Reactant 2

HCID 192826alpha-Maltotriose2×HCID 53810987GlyTouCan:G55967QW

Product 3

HCID 6255beta-MaltoseHCID 192826alpha-MaltotrioseHCID 53810987GlyTouCan:G55967QW

Reagents, catalysts & solvents 1

HCID 175AcetateSolvent

Procedure

The α-isomaltosylglucosaccharide-forming enzyme activity can be assayed as follows: Add 0.5 ml of an enzymatic solution to 0.5 ml of a substrate solution obtained by dissolving maltotriose in 100 mM acetate buffer (pH 6.0) to give a concentration of 2% (w/v) is added, incubate the mixture at 35° C. for 60 min to proceed the formation reaction of isomaltosylmaltose from maltotriose. In the reaction system, maltose is formed together with isomaltosylmaltose from maltotriose. Thereafter, boil the reaction mixture for 10 min to suspend the enzymatic reaction. Subject the resulting mixture to-conventional HPLC for detecting and quantifying maltose formed in the reaction mixture to quantify the maltose. In the present invention, one unit activity of the α-isomaltosylglucosaccharide-forming enzyme is defined as the enzyme amount that forms one micromole of maltose per minute under the above enzymatic reaction conditions.

Workup

  1. customobtained
  2. customto give a concentration of 2% (w/v)
  3. additionis added

Sources & evidence

ORD record
ord-7de52d8eb44c4ecfb28a3b0c2388038b
Source dataset
uspto-grants-2007_05
Patent number
US07223570B2

Reaction notes

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