alpha-Maltotriose → beta-Maltose

Participants

Reaction Steps

The α-isomaltosylglucosaccharide-forming enzyme activity can be assayed as follows: Add 0.5 ml of an enzymatic solution to 0.5 ml of a substrate solution obtained by dissolving maltotriose in 100 mM acetate buffer (pH 6.0) to give a concentration of 2% (w/v) is added, incubate the mixture at 35° C. for 60 min to proceed the formation reaction of isomaltosylmaltose from maltotriose. In the reaction system, maltose is formed together with isomaltosylmaltose from maltotriose. Thereafter, boil the reaction mixture for 10 min to suspend the enzymatic reaction. Subject the resulting mixture to-conventional HPLC for detecting and quantifying maltose formed in the reaction mixture to quantify the maltose. In the present invention, one unit activity of the α-isomaltosylglucosaccharide-forming enzyme is defined as the enzyme amount that forms one micromole of maltose per minute under the above enzymatic reaction conditions.

Provenance & Evidence