Sodium Bicarbonate → HCID 88116952, compound

Participants

Reaction Steps

(7R)-7-[2-(2-Amino-5-chlorothiazol-4-yl)-2-hydroxyiminoacetylamino]-3-(5-amino-[1,3,4]thiadiazol-2-ylsulfanyl)-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid (85 mg, 0.11 mmol) was dissolved in water (2.0 mL) and the pH was adjusted to 6.0 with 1.0 M aqueous sodium bicarbonate. The solution was loaded onto an HP20 column and inorganics were removed by washing the column thoroughly with water. The title compound was isolated by eluting with 2:1 acetonitrile/water, removing acetonitrile under reduced pressure and lyophilizing the remaining mixture (60 mg, 63%).

Provenance & Evidence