Sodium Hydroxide → L-Lysine

Participants

Reaction Conditions

Reaction Steps

A medium containing 40 g/L of glucose, 0.6 g/L (as nitrogen) of soybean protein hydrolysate, 1 g/L of KH2PO4, 5.6 g/L of NaOH, 1.0 g/L of ammonium sulfate, 10 mg/L of FeSO4.7H2O and 10 mg/L of MnSO4.4H2O (pH 6.0) was introduced into 1-L volume small glass fermentation tank in a volume of 300 mL and sterilized by heating at 120° C. for 20 minutes. After the fermentation tank was cooled to 31.5° C., 5 platinum loops of Escherichia coli W3110(tyrA)/pCABD2 (International Patent Publication WO95/16042) preliminarily grown on an LB plate for 24 hours was inoculated to the medium and cultured at 37° C. and pH 6.7 for 24 hours with sufficient aeration and stirring.

Provenance & Evidence