Sodium Hydroxide → L-Lysine

HRID 1362820Notes

Reaction equation

HRID 1362820 reaction equation
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])O)O)O)O.OP([O-])(O)=O.[K+].[OH-:19].[Na+].S([O-])([O-])(=O)=O.[NH4+:26].[NH4+:27]>[Pt]>[NH2:26][C@H:9]([C:11]([OH:12])=[O:19])[CH2:7][CH2:5][CH2:3][CH2:2][NH2:27]

Participants

6

Reactant 4

HCID 14798Sodium HydroxideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 516951Monobasic potassium phosphateHCID 6097028Ammonium Sulfate

Product 1

HCID 5962L-Lysine

Reagents, catalysts & solvents 1

HCID 23939PlatinumCatalyst

Conditions

Temperature
120 °C

Procedure

A medium containing 40 g/L of glucose, 0.6 g/L (as nitrogen) of soybean protein hydrolysate, 1 g/L of KH2PO4, 5.6 g/L of NaOH, 1.0 g/L of ammonium sulfate, 10 mg/L of FeSO4.7H2O and 10 mg/L of MnSO4.4H2O (pH 6.0) was introduced into 1-L volume small glass fermentation tank in a volume of 300 mL and sterilized by heating at 120° C. for 20 minutes. After the fermentation tank was cooled to 31.5° C., 5 platinum loops of Escherichia coli W3110(tyrA)/pCABD2 (International Patent Publication WO95/16042) preliminarily grown on an LB plate for 24 hours was inoculated to the medium and cultured at 37° C. and pH 6.7 for 24 hours with sufficient aeration and stirring.

Workup

  1. temperatureAfter the fermentation tank was cooled to 31.5° C.
  2. customcultured at 37° C.
  3. waitpH 6.7 for 24 hours with sufficient aeration

Sources & evidence

ORD record
ord-463b689b65d84c9687bdc3f3ab561ecc
Source dataset
uspto-grants-2011_02
Patent number
US07883878B2

Reaction notes

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