反应详情

N-Cyclohexyl-2-(ethoxymethyl)-5-oxido-1H-imidazo[4,5-c]quinolin-1-amine (0.425 g, 1.25 mmol) was placed in a 100 mL round bottom flask and dissolved in dichloromethane (20 mL). Ammonium hydroxide solution (10 mL) was added and the mixture was stirred vigorously. The stirred mixture was chilled in an ice water bath. Para-toluenesulfonyl chloride (0.250 g, 1.31 mmol) was added over 5 min. After 30 min of stirring at 0° C. TLC (SiO2, 95:5 chloroform:methanol) showed complete conversion. The mixture was warmed to room temperature and then diluted with dichloromethane (25 mL) and water (10 mL). The mixture was transferred to a separatory funnel and the phases separated. The organic portion was washed sequentially with 2% sodium carbonate solution (15 mL), water (15 mL) and brine (15 mL), dried over Na2SO4, filtered and then concentrated to yield an orange/tan foamy solid. The material was purified by column chromatography (40 g SiO2, 95:5 chloroform:methanol) to yield the product as an off white solid. The off-white solid was dissolved in 3 mL of a 9:1 chloroform:methanol mixture. A small spatula tip full of activated carbon (DARCO G 60–100 mesh) was added and the mixture was stirred at room temperature for 3 h. The mixture was filtered through a short column of SiO2 (5 g) eluting with 9:1 chloroform:methanol. The filtrate was concentrated to yield a glassy solid. The glassy solid was triturated in 15 mL diethyl ether for 2 h to provide a white solid. The solid was collected by vacuum filtration and rinsed with diethyl ether. The solid was dried in a vacuum oven (70° C.) to yield 0.062 g of N1-cyclohexyl-2-(ethoxymethyl)-1H-imidazo[4,5-c]quinoline-1,4-diamine. mp 143–145° C.; 1H NMR (300 MHz, DMSO-d6) δ 8.61 (dd, J=8.1, 1.1 Hz, 1H), 7.58 (dd, J=8.3, 0.9 Hz, 1H), 7.46–7.38 (m, 1H), 7.28–7.21 (m, 1H), 6.99 (d, J=1.9 Hz, 1H), 6.69 (s, 2H), 4.77 (s, 2H), 3.63 (q, J=7.0 Hz, 2H), 3.32–3.23 (m, 1H), 1.71–1.52 (m, 5H), 1.30–1.05 (m, 8H); 13C NMR (75 MHz, DMSO-d6) δ; MS m/z 152.1, 150.3, 145.0, 133.4, 127.4, 125.8, 123.9, 121.6, 121.1, 115.0, 65.8, 63.1, 59.8, 30.9, 25.8, 24.3, 15.4; MS m/z 340 (M+H)+; Anal. Calcd for C19H25N5O: C, 67.23; H, 7.42; N, 20.63; Found: C, 67.32; H, 7.37; N, 20.55.

1. temperatureThe stirred mixture was chilled in an ice water bath
2. stirringAfter 30 min of stirring at 0° C
3. customThe mixture was transferred to a separatory funnel
4. customthe phases separated
5. washThe organic portion was washed sequentially with 2% sodium carbonate solution (15 mL), water (15 mL) and brine (15 mL)
6. dry with materialdried over Na2SO4
7. filtrationfiltered
8. concentrationconcentrated
9. customto yield an orange/tan foamy solid
10. customThe material was purified by column chromatography (40 g SiO2, 95:5 chloroform:methanol)
11. customto yield the product as an off white solid
12. additionA small spatula tip full of activated carbon (DARCO G 60–100 mesh) was added
13. stirringthe mixture was stirred at room temperature for 3 h
14. filtrationThe mixture was filtered through a short column of SiO2 (5 g)
15. washeluting with 9:1 chloroform
16. concentrationThe filtrate was concentrated
17. customto yield a glassy solid
18. customThe glassy solid was triturated in 15 mL diethyl ether for 2 h
19. customto provide a white solid
20. filtrationThe solid was collected by vacuum filtration
21. washrinsed with diethyl ether
22. customThe solid was dried in a vacuum oven (70° C.)