反应详情

温度

30 °C

Examples 2t and 2u, Step 4: A sample of benzyl (S)-1-((1S,2R,4R)-4-(tert-butyldimethylsilyloxy)-2-propylcyclohexylamino)-4-(methylthio)-1-oxobutan-2-ylcarbamate (4.0 g, 7.45 mmol) was dissolved in iodomethane (8 mL) and stirred at 30° C. for 3 days. The solution was concentrated in vacuo. The residue was dissolved in methylene chloride and the resultant solution was concentrated in vacuo again; this procedure was repeated twice before the residue was placed under high vacuum for 4 h. The resultant pale yellow foam solid was dissolved in THF (40 mL) and the resultant solution was cooled to 0° C. before being treated with sodium hydride (0.9 g, 37 mmol) in one portion. The mixture was slowly warmed to 30° C. and stirred for 12 h before being quenched with saturated ammonium chloride. The organic layer was separated and the aqueous layer was extracted with EtOAc. The combined organic phases were washed with brine, dried, filtered, and concentrated in vacuo. The residue was purified by flash chromatography to afford benzyl (S)-1-((1S,2R,4R)-4-(tert-butyldimethylsilyloxy)-2-propylcyclohexyl)-2-oxopyrrolidin-3-ylcarbamate (0.9 g). MS found: (M+H)+=489.2.

1. concentrationThe solution was concentrated in vacuo
2. dissolutionThe residue was dissolved in methylene chloride
3. concentrationthe resultant solution was concentrated in vacuo again
4. waitwas placed under high vacuum for 4 h
5. dissolutionThe resultant pale yellow foam solid was dissolved in THF (40 mL)
6. temperaturethe resultant solution was cooled to 0° C.
7. temperatureThe mixture was slowly warmed to 30° C.
8. stirringstirred for 12 h
9. custombefore being quenched with saturated ammonium chloride
10. customThe organic layer was separated
11. extractionthe aqueous layer was extracted with EtOAc
12. washThe combined organic phases were washed with brine
13. customdried
14. filtrationfiltered
15. concentrationconcentrated in vacuo
16. customThe residue was purified by flash chromatography