N,N',N''-Triacetylchitotriose → N-Acetylchitohexaose

Participants

Reaction Steps

The combined effluent fractions were freeze-dried to give 36 g of a solid mixture comprising tetra-N-acetylchitotetraose (NACOS-4), penta-N-acetyl-chitopentaose (NACOS-5) and hexa-N-acetyl-chitohexaose (NACOS-6), together with minor proportions of N-acetylglucosamine, di-N-acetyl-chitobiose, tri-N-acetyl-chitotriose. This mixture comprising the water-soluble chitin-oligomers was then chromatographed by a high pressure liquid chromatography on a column of μ-Bondapack CH (a product of Waters Company, U.S.A.) as eluted with methylnitrile-water (70:30 by volume), to effect the isolation of the chitin-oligomers from each other, affording 3.10 g of hexa-N-acetylchitohexaose having an [α]D of -11.2° and a decomposition temperature of 300° C., 7.6 g of penta-N-acetyl-chitopentaose having an [α]D of -9.0° and a decompsition temperature of 304° C.; and 8.1 g of tetra-N-acetyl-chitotetraose having an [α]D of -4.2° and a decomposition temperature of 299° C.

Provenance & Evidence