反应详情

To a solution of 2 ml 15 mM Bz-Ala-Leu-NH2 in 0.1M KCl-1 mM EDTA, pH 9.7, 25° C. and 10% dimethyl formamide was added 2 mg CPD-Y. The reaction course as shown on FIG. 5 was followed by HPLC as described in example 1. It is seen that Bz-Ala-Leu-NH2 after 20 minutes was completely converted to about 68% Bz-Ala-Leu-OH and 32% Bz-Ala-OH. Amino acid analysis on the reaction mixture showed that the Bz-Ala-OH was mainly formed by cleavage of Leu-NH2 from Bz-Ala-Leu-NH2.

1. customafter 20 minutes