反应详情

Food is withheld from the animals for from 20 to 22 hours prior to the operation. They are allowed to take water ad lib. The operation and the perfusion of the liver are carried out using the technique of R. Scholz et al. [Eur. J. Biochem. 38 (1973) 64 to 72]. The perfusion liquid used is Krebs-Henseleit bicarbonate buffer (pH 7.4), which is saturated with an oxygen/carbon dioxide mixture (95/5) and contains 1.6 mmol/liter of L-lactate and 0.2 mmol/liter of pyruvate. The perfusion liquid is pumped into the liver via a cannula inserted into the portal vein. The effluent perfusion liquid is collected via a cannula inserted into the Vena cava. The liver is perfused for about 2 hours. The test compounds are infused for 16 minutes each from the 32nd to the 80th minute after the perfusion in increasing concentrations (0.02 to 1.00 mmol/liter).

1. customis withheld from the animals for from 20 to 22 hours
2. customThe effluent perfusion liquid is collected via a cannula
3. waitThe test compounds are infused for 16 minutes
4. temperatureeach from the 32nd to the 80th minute after the perfusion in increasing
5. concentrationconcentrations (0.02 to 1.00 mmol/liter)