Magnesium Chloride → L-Aspartic Acid

HRID 1390193Notes

Reaction equation

[C:1]([O-:8])(=[O:7])/[CH:2]=[CH:3]/[C:4]([O-:6])=[O:5].[NH4+:9].[NH4+].[Cl-].[Mg+2].[Cl-]>>[NH2:9][C@H:2]([C:1]([OH:8])=[O:7])[CH2:3][C:4]([OH:6])=[O:5]

Participants

Reactant 2

HCID 5360315Magnesium Chloride

HCID 9793847Ammonium fumarate2×

Product 1

HCID 5960L-Aspartic Acid

Conditions

Temperature: 37 °C

Procedure

[Aspartase activity is indicated in terms of micromoles of L-aspartic acid which are produced by reaction with ammonium fumarate at 37° C. at pH 8.5. The reaction with ammonium fumarate is conducted by adding 2 g of the immobilized aspartase preparation to 30 ml of an aqueous 1 M ammonium fumarate solution (pH 8.5) containing 1 mM magnesium chloride, and then shaking the mixture at 37° C. for one hour. The amount of L-aspartic acid produced is bioassayed by using leuconostoc mesenterioides P-60(J. Biol. Chem., 172, 15(1948).]

Workup

additionby adding

Sources & evidence

ORD record: ord-3fc7f1d0179d45f1a9263917c3c57b59
Source dataset: uspto-grants-1979_02
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04138292

Reaction notes

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