Magnesium Chloride → L-Aspartic Acid

Participants

Reaction Conditions

Reaction Steps

[Aspartase activity is indicated in terms of micromoles of L-aspartic acid which are produced by reaction with ammonium fumarate at 37° C. at pH 8.5. The reaction with ammonium fumarate is conducted by adding 2 g of the immobilized aspartase preparation to 30 ml of an aqueous 1 M ammonium fumarate solution (pH 8.5) containing 1 mM magnesium chloride, and then shaking the mixture at 37° C. for one hour. The amount of L-aspartic acid produced is bioassayed by using leuconostoc mesenterioides P-60(J. Biol. Chem., 172, 15(1948).]

Provenance & Evidence