Sulfuric acid magnesium salt (1:1) → D-(-)-Fructose

Participants

Reaction Conditions

Reaction Steps

[One unit of glucose isomerase is defined as the enzymatic activity which produces one micromole of fructose by reaction with glucose at 37° C. at pH 7.0 for one minute. The reaction with glucose is conducted by adding 2 g of the immobilized glucose isomerase preparation to 20 ml of an aqueous 0.1 M glucose solution (pH 7.0) containing 0.01 M of magnesium sulfate, 1 mM cobaltous chloride and 0.1 M sodium sulfite, and then shaking the mixture at 37° C. for one hour. The amount of fructose produced is measured by the cysteinecarbazole-sulfuric acid method.]

Provenance & Evidence