Sulfuric acid magnesium salt (1:1) → D-(-)-Fructose
Reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].S([O-])([O-])(=O)=O.[Mg+2].S([O-])([O-])=O.[Na+].[Na+]>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]Participants
4
Conditions
- Temperature
- 37 °C
Procedure
[One unit of glucose isomerase is defined as the enzymatic activity which produces one micromole of fructose by reaction with glucose at 37° C. at pH 7.0 for one minute. The reaction with glucose is conducted by adding 2 g of the immobilized glucose isomerase preparation to 20 ml of an aqueous 0.1 M glucose solution (pH 7.0) containing 0.01 M of magnesium sulfate, 1 mM cobaltous chloride and 0.1 M sodium sulfite, and then shaking the mixture at 37° C. for one hour. The amount of fructose produced is measured by the cysteinecarbazole-sulfuric acid method.]
Workup
- additionby adding
Sources & evidence
- ORD record
- ord-cffbcd144d0541d19b126fa2de9bf69f
- Source dataset
- uspto-grants-1979_02
- Patent number
- US04138292
Reaction notes
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