L-Alanine → L-Aspartic Acid
Reaction equation
[NH2:1][C@H:2]([C:7]([O-:9])=[O:8])[CH2:3][C:4]([O-:6])=[O:5].N[C@H](C(O)=O)C>>[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][C:4]([OH:6])=[O:5]Participants
3
Procedure
[Aspartate β-decarboxylase activity is indicated in terms of micromoles of L-alanine which are produced by reaction with 0.2 M L-aspartic acid. The reaction with L-aspartic acid is conducted by adding 12 g of the immobilized preparation to 30 ml of an aqueous 0.2 M ammonium L-asparaginate solution (pH 5.5) containing 10-4M pyridoxal phosphate, and shaking the mixture at 37° C. for one hour. The amount of L-alanine produced is bioassayed by using Leuconostoc mesenterioides P-60.]
Sources & evidence
- ORD record
- ord-bde0aeee52c749cda8d2ac4744fdfc22
- Source dataset
- uspto-grants-1979_02
- Patent number
- US04138292
Reaction notes
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