L-Alanine → L-Aspartic Acid

Participants

Reaction Steps

[Aspartate β-decarboxylase activity is indicated in terms of micromoles of L-alanine which are produced by reaction with 0.2 M L-aspartic acid. The reaction with L-aspartic acid is conducted by adding 12 g of the immobilized preparation to 30 ml of an aqueous 0.2 M ammonium L-asparaginate solution (pH 5.5) containing 10-4M pyridoxal phosphate, and shaking the mixture at 37° C. for one hour. The amount of L-alanine produced is bioassayed by using Leuconostoc mesenterioides P-60.]

Provenance & Evidence