trans-Urocanic acid → HCID 178419078, compound

HRID 1390197Notes

Reaction equation

HRID 1390197 reaction equation
N.[NH2:2][C@H:3]([C:10]([OH:12])=[O:11])[CH2:4][C:5]1[N:9]=[CH:8][NH:7][CH:6]=1.C1N=CNC=1/C=C/C(O)=O>>[NH2:2][C@H:3]([C:10]([OH:12])=[O:11])[CH2:4][C:5]1[N:9]=[CH:8][NH:7][CH:6]=1

Participants

3

Reactant 2

HCID 736715trans-Urocanic acidHCID 178514376HCID 178514376, compound

Product 1

HCID 178419078HCID 178419078, compound

Procedure

[L-histidine ammonia-lyase activity is indicated in terms of micromoles of urocanic acid which are produced by reaction with L-histidine. The reaction with L-histidine is conducted by adding 10 g of the immobilized preparation to 30 ml of an aqueous 0.25 M L-histidine solution (pH 9.0), and shaking the mixture at 37° C. for one hour. The amount of urocanic acid produced in assayed colorimetrically at 277 nm (molecular extinction coefficient = 1.88 × 10-4 (pH 7.4)).]

Sources & evidence

ORD record
ord-a2d9f602f5d944279af9f3109bd45853
Source dataset
uspto-grants-1979_02
Patent number
US04138292

Reaction notes

Loading…