L-(-)-Sorbose → L-Ascorbic Acid

Participants

Reaction Steps

3 ml of the seed culture were transferred into 500 ml Erlenmeyer flasks containing 50 ml of the production medium containing 8.0% L-sorbose, 5.0% baker's yeast, 0.05% glycerol, 0.25% MgSO4.7H2O, 3.0% corn steep liquor, 1.5% CaCO3 and 0.15% antifoam. The cultivation was carried out at 30° C. with 180 rpm for 20 h on a rotary shaker. As a result, 407.1 mg/L of vitamin C was produced.

Provenance & Evidence