D-(-)-Fructose → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 1395697Notes

Reaction equation

C1C2NC3C(=CC=CC=3)C=2C=CC=1.N[C@H](C(O)=O)CS.[OH:21][CH2:22][C:23]([C@H:25]([C@@H:27]([C@@H:29]([CH2:31][OH:32])[OH:30])[OH:28])[OH:26])=[O:24]>>[O:21]=[CH:22][C@@H:23]([C@H:25]([C@@H:27]([C@@H:29]([CH2:31][OH:32])[OH:30])[OH:28])[OH:26])[OH:24]

Participants

Reactant 2

HCID 5984D-(-)-Fructose

HCID 22825735HCID 22825735, compound

Product 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Procedure

A mixture of 0.2 ml of a 1 M aqueous D-glucose solution, 0.2 ml of a 0.05 M aqueous MgSO4.7H2O solution, 0.2 ml of a 0.5 M aqueous phosphate buffer solution (pH=7.2) and a given amount of aqueous glucose isomerase extract is diluted with water to make 2 ml. The resulting mixture is maintained at a temperature of 70° C. for 60 minutes to effect the glucose isomerization which is terminated by the addition of 2 ml of 0.5 M perchloric acid. The amount of fructose produced is determined by cystein carbazole method. The value obtained by dividing the amount of fructose produced by the amount of aqueous glucose isomerase extract is the activity, the unit of which is expressed by the abbreviation "U".

Workup

customThe value obtained

Sources & evidence

ORD record: ord-32312c900bc94962a96ae482c917bbf6
Source dataset: uspto-grants-1980_05
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04205127

Reaction notes

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