Hydrochloric Acid → HCID 178455969, compound

Participants

Reaction Steps

29.4 g of t-butoxycarbonylisoleucylhistidine methyl ester is dissolved in 150 ml of methanol. To this solution is added 308 ml of 1 N lithium hydroxide. The reaction mixture is stirred for three hours, then neutralized with 1 N hydrochloride acid to pH 3. The solvent is removed by vacuum and the residue is dissolved in N,N-dimethylformamide. The solution is filtered and the solvent removed by vacuum. The residue is dissolved in methanol/2-propanol. The alcohol solution is added to 2 liters of cold rapidly stirring ethyl ether. The white precipitate is filtered and dried in vacuo to afford t-butoxycarbonylisoleucylhistidine hydrochloride.

Provenance & Evidence