反应详情

29.4 g of t-butoxycarbonylisoleucylhistidine methyl ester is dissolved in 150 ml of methanol. To this solution is added 308 ml of 1 N lithium hydroxide. The reaction mixture is stirred for three hours, then neutralized with 1 N hydrochloride acid to pH 3. The solvent is removed by vacuum and the residue is dissolved in N,N-dimethylformamide. The solution is filtered and the solvent removed by vacuum. The residue is dissolved in methanol/2-propanol. The alcohol solution is added to 2 liters of cold rapidly stirring ethyl ether. The white precipitate is filtered and dried in vacuo to afford t-butoxycarbonylisoleucylhistidine hydrochloride.

1. customThe solvent is removed by vacuum
2. dissolutionthe residue is dissolved in N,N-dimethylformamide
3. filtrationThe solution is filtered
4. customthe solvent removed by vacuum
5. dissolutionThe residue is dissolved in methanol/2-propanol
6. additionThe alcohol solution is added to 2 liters of cold rapidly stirring ethyl ether
7. filtrationThe white precipitate is filtered
8. customdried in vacuo