Water → Tris(hydroxymethyl)aminomethane
Reaction equation
[NH2:1][C@H:2]([C:8]([OH:10])=O)[CH2:3]CC(=O)O.N[C@H](C(O)=O)C[C:14](=O)[OH:15].[OH2:20].[B:21]([O-:24])([O-:23])[O-:22]>>[B:21]([O-:24])([O-:23])[O-:22].[CH2:14]([OH:15])[C:2]([NH2:1])([CH2:3][OH:20])[CH2:8][OH:10]Participants
6
Procedure
Poly(D/L Glu-DET)/DNA transfection complexes were prepared by initially adding equal volumes of Poly(D/L Glu-DET) solution (100 μL dissolved in either dH2O or 5% BSA-50 mM Borate pH 8.3) and plasmid DNA solution (20 μg dissolved in 100 μL in either dH2O or 5% BSA-50 mM Borate pH 8.3) at N:P ratio 10. Polymer was added to DNA solution, for a final volume of 200 μL, and incubated at room temperature for at least 15 min to allow polyplex formation. Transfection polyplexes were formed by incubating 200 μL of Poly(D/L Asp-DET)/DNA N:P 10 polyplexes with 300 μL of either H2O, 5% BSA-50 mM Borate pH 8.3, or 5% BSA-10 mM Tris pH 8 for 15 min at room temperature to give a final volume of 500 μL for all samples. (See FIG. 1 for schematic).
Workup
- waitfor a final volume of 200 μL, and incubated at room temperature for at least 15 min
- customTransfection polyplexes were formed
Sources & evidence
- ORD record
- ord-1046f6c60a674177b76aefa46b6aab4d
- Source dataset
- uspto-grants-2014_12
- Patent number
- US08921111B2
Reaction notes
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