Glutaraldehyde → HCID 86754116, compound

The enzyme gelling solution for infiltration into the membranes was formulated with Glucose Oxidase, Type VII from Aspergillus Niger, EC 1.1.3.4 (Sigma Chemical Co.) and Bovine Serum Albumin, Fraction V Powder (Sigma Chemical Co.). About 0.1000 grams of Glucose Oxidase and 0.400 grams of Bovine Serum Albumin are mixed together in a 0.20 M Sodium Acetate (pH=5.00) (Sigma Chemical Co.) buffer, until they both dissolve in the acetate buffer (volume=7.5 ml). In a separate vial, a solution of 1.0 ml 25% Glutaraldehyde, Grade 1 (Sigma Chemical Co.) is mixed with 9.0 ml of acetate buffer (pH=5.00), which results in a 2.5% Glutaraldehyde/acetate buffer. A 2.5 ml aliquot of this 2.5% Glutaraldehyde/acetate buffer is removed and set aside for use later in crosslinking the enzyme gel in the pores of the modified White Silon #320 membrane. About 2.5 ml of the Glucose Oxidase/Bovine Serum Albumin solution is placed on the modified White Silon #320 membrane (one of the 2" by 3" strips of membrane on a glass plate) via a pipette. These drops wicked into the membrane and the solution was uniformly dispersed in the membrane by gently rubbing the membrane with a smooth blunt spatula. A visual judgement is made of the membrane after the Glucose Oxidase/Bovine Serum Albumin (GOD/BSA) is allowed to dry for about 30 minutes. If the solution of GOD/BSA has penetrated the pores of the membrane and evenly deposited within the pores the membrane will look a yellow translucent color. If there are gaps or incomplete deposition there will be spots of the membrane, which will not be colored yellow. After this visual inspection, about 0.8 ml of the 2.5% Glutaraldehyde/acetate buffer was added to the membrane as droplets and this solution was spread over the entire membrane area, in order to ensure even distribution of the cross-linking and immobilizing agent, Glutaraldehyde. The glass plate holding the GOD/BSA embedded White Silon #320 membrane was placed in a plastic zip lock bag (Glad), with a small amount of the 2.5% Glutaraldehyde solution in an open vial and left enclosed in this plastic bag for a period of 60 hours. The fully crosslinked and immobilized enzyme gel membrane was removed from this plastic bag and placed in another bag, which contained Gomori Buffer soaked cotton gauzes and the top of the bag was zipped shut. The buffer soaked gauzes maintained a humid environment for the enzyme gel membrane in the closed plastic bag, and the closed bag was marked with the appropriate date and laboratory notes and placed in a refrigerator for storage.

ORD record

ord-b3a4841565b2478e8ad0e3142b21300c

Source dataset

uspto-grants-1999_06

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05914026