Ammonia → L-Aspartic Acid

HRID 1416847Notes

Reaction equation

HRID 1416847 reaction equation
P([O-])([O-])([O-])=O.[C:6]([OH:13])(=[O:12])/[CH:7]=[CH:8]/[C:9]([OH:11])=[O:10].[NH3:14]>>[NH2:14][C@H:7]([C:6]([OH:13])=[O:12])[CH2:8][C:9]([OH:11])=[O:10]

Participants

4

Reactant 3

HCID 222AmmoniaHCID 1061PhosphateHCID 444972Fumaric Acid

Product 1

HCID 5960L-Aspartic Acid

Procedure

The culture of H-9183 strain as obtained in the controlled aeration condition in Example 1 was centrifuged to isolate the cells. The cells were suspended in a phosphate buffer (0.01 M potassium phosphate; pH 7.4), and homogenized along with glass beads to disrupt them into an extract. The extract was passed through a column filled with a resin, Duolite A-7 to thereby make the protein existing in the extract adsorbed by the resin. Then, an aqueous solution of fumaric acid (150 g/liter; adjusted the pH at 8.5 with aqueous ammonia) was passed through the column at 37° C. to thereby obtain a solution of L-aspartic acid (171 g/liter). The pH of the solution(1 liter) was adjusted to 2.7 with sulfuric acid, and 161 g of L-aspartic acid crystals was obtained.

Workup

  1. customThe culture of H-9183 strain as obtained in the controlled aeration condition in Example 1
  2. customto isolate the cells
  3. additionfilled with a resin, Duolite A-7
  4. customwas passed through the column at 37° C.

Sources & evidence

ORD record
ord-0e48519e649943c688cbbc2df13b1ccc
Source dataset
uspto-grants-1999_06
Patent number
US05916782

Reaction notes

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