Ammonia → L-Aspartic Acid

Participants

Reaction Steps

The culture of H-9183 strain as obtained in the controlled aeration condition in Example 1 was centrifuged to isolate the cells. The cells were suspended in a phosphate buffer (0.01 M potassium phosphate; pH 7.4), and homogenized along with glass beads to disrupt them into an extract. The extract was passed through a column filled with a resin, Duolite A-7 to thereby make the protein existing in the extract adsorbed by the resin. Then, an aqueous solution of fumaric acid (150 g/liter; adjusted the pH at 8.5 with aqueous ammonia) was passed through the column at 37° C. to thereby obtain a solution of L-aspartic acid (171 g/liter). The pH of the solution(1 liter) was adjusted to 2.7 with sulfuric acid, and 161 g of L-aspartic acid crystals was obtained.

Provenance & Evidence