Pyridoxal 5'-phosphate → D-Tryptophan

Participants

Reaction Conditions

Reaction Steps

To 9 ml of the culture medium obtained in Examples 1, 2 and 3 were added 1 ml of 1 M potassium phosphate buffer (pH 8.0), 0.025 g of D,L-tryptophan, 50 μl of 10 mM pyridoxal 5'-phosphate. The mixture was allowed to react with shaking at 37° C. for 20 hours. The optical purity and amount of tryptophan after the reaction were determined with Crown Pak CR(+) (product of Daicel Chemical Industries, Ltd.) using perchloric acid solution (pH 2.0) as eluent at 40° C. The results of the reaction were shown in Table 1. (In Table 1, data of Cellulomonas sp., Corynebacterium ammoniagenes, Providencia rettgeri, and Serratia grimesii were obtained in this Example.)

Provenance & Evidence