反应详情

The same operation as in Example (90g) was performed using ethyl 2-(5-{[(4-chloro-5-ethyl-1H-imidazol-2-yl)carbonyl]amino}-1,3-dihydro-2H-isoindol-2-yl)-1,3-thiazole-5-carboxylate obtained in Example (92b) (92 mg, 0.21 mmol), 2 N lithium hydroxide (3 mL, 6 mmol), methanol (5 mL) and dichloromethane (5 mL). Purification by reverse phase chromatography (elution solvent: acetonitrile/water=0/1, 1/1, 4/1) gave 49 mg of the title compound as a light brown solid (57%).

1. customPurification
2. washby reverse phase chromatography (elution solvent: acetonitrile/water=0/1, 1/1, 4/1)