HRID14353

反应详情

EQUATION

反应方程式

REACTANTS

反应物

HCID19660

Carbonate

CO3-2

结构：O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CCC1=O

HCID83874

N-{6-[(2,5-Dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-5-(2-hydroxy-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl)pentanimidic acid

C20H30N4O6S

2 次

PRODUCTS

生成物

结构：O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21

HCID171548

未命名化合物

PROCEDURE

实验过程

Anti-rabbit IgG monoclonal antibodies (2B9 5 μg/mL) dissolved in 0.1 M carbonate buffer (pH 8.5) and NHS-LC-BIOTIN (PIERCE, 25 μg/mL) were mixed and stirred at room temperature for four hours using a stirrer. IgG and NHS-LC-BIOTIN were mixed at a mole ratio of 1:60. After stirring, this solution was dialyzed against physiological phosphate buffered saline (PBS) to obtain biotin-labeled antibodies.

WORKUP

后处理

stirringAfter stirring
customthis solution was dialyzed against physiological phosphate buffered saline (PBS)