Uridine Diphosphate → N-Acetyl-D-Glucosamine

Participants

Role	Name	HCID	Charge
Reactant	Uridine Diphosphate	HCID 6031	—
Reactant	Bucladesine	HCID 9687	—
Reactant	Udp-Galactose	HCID 18068	—
Reactant	X 100	HCID 19153	—
Reactant	Phorbol 12-myristate 13-acetate	HCID 27924	—
Reactant	8-bromo-cAMP	HCID 32014	—
Reactant	N-acetyl-alpha-D-galactosamine	HCID 84265	—
Reactant	Uridine-Diphosphate-N-Acetylglucosamine	HCID 445675	—
Reactant	Monobasic potassium phosphate	HCID 516951	—
Reactant	(2R,3S,4S,5R)-2,3,4,5,6-pentahydroxyhexanal	HCID 3037556	—
Reactant	beta-D-Gal-(1->3)-alpha-D-GalNAc	HCID 5288347	—
Reactant	HCID 178513008	HCID 178513008	—
Solvent	Acetonitrile	HCID 6342	—
Product	N-Acetyl-D-Glucosamine	HCID 439174	—

Reaction Steps

Galβ1-3GalNAcα-pNp and GalNAcα-pNp, H-7, H-8 and H-1004 were purchased from Toronto Research Chemicals, Toronto, Canada, UDP-Gal, UDP-GlcNAc, bovine milk β1-4Gal-T (defined as 5-15 units/mg in the presence of lactalbumin), buffer salts and Triton X-100, cholera toxin, dibutyryl cAMP, 8-bromo-cAMP and phorbol 12-myristate 13-acetate (PMA) and TDA were obtained from Sigma. HPLC grade KH2PO4 and acetonitrile were obtained from Fisher Scientific. UDP-6- 3H!-galactose (18.9 Ci/mmol) and UDP-6- 3H!-N-acetylglucosamine (26.8 Ci/mmol) were provided by Amersham and NEN, respectively. C18 Sep-Pak were from Millipore-Waters. Actinomycin D and cycloheximide were purchased from Calbiochem. UDP-6- 3H!-galactose (18.9 Ci/mmole) was purchased from Amersham; dimethysulfoxide (DMSO) from BDH; all-trans-retinoic acid and sodium butyrate from Aldrich; α-modified Eagle's minimum essential medium (α-MEM) and fetal calf serum from Gibco.

Provenance & Evidence

DOI	10.6084/m9.figshare.5104873.v1
Patent number	US05501957
Source dataset	uspto-grants-1996_03
ORD ID	ord-680e10130a674bda9d787e67e62d5f31
Source note	uspto-grants-1996_03