HuagongsheHuagongshe Chemistry Print Report · For research reference only
Creatine → Camp
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Creatine | HCID 586 | — |
| Reactant | 3-Isobutyl-1-methylxanthine | HCID 3758 | — |
| Reactant | 5'-Atp | HCID 5957 | — |
| Reactant | 2-[Carbamimidoyl(methyl)amino]acetic acid;phosphoric acid | HCID 18629879 | — |
| Reactant | Guanosine Triphosphate | HCID 135398633 | — |
| Solvent | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| Product | Camp | HCID 6076 | — |
Reaction Steps
Membranes are incubated in 25 mM Tris (pH 7.6), 0.2% BSA, 2.6 mM Mg, 0.8 mM ATP, 0.1 mM GTP, 5 mM creatine phosphate, 50 U/ml creatine kinase, 0.2 mM IBMX at 32° C. Prospective ligands are added and incubation is continued for another 15 minutes. The amount of cAMP produced is assayed using an fluorescent immunoassay method.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US07655396B1 |
| Source dataset | uspto-grants-2010_02 |
| ORD ID | ord-7ecdaa8541524bb59512d24ab4b045aa |
| Source note | uspto-grants-2010_02 |