Bromoacetyl bromide → HCID 136378435, compound

Participants

Reaction Steps

A porphyrin (5-(3-aminophenyl)-10,15,20-triphenylporphyrin is derivatized by reacting with excess bromoacetyl-bromide (dissolved in acetone containing sodium carbonate) to form (5-(3-bromoacetamidophenyl)-10,15,20-triphenylporphyrin. 10 μmol of the derivatized porphyrin and 10 mg of the scFv-4-helix bundle-cys are dissolved separately in 20 ml of dry dimethyl formamide. These two solutions are added over a period of 3 hours to 50 ml dry dimethyl formamide containing 25 mg sodium carbonate. These reactions are performed in the dark, under argon. The final product is scFv-4-helix bundle-cys with optimally conjugated porphyrins (scFv-4-helix bundle-cys/porphyrin). The scFv-4-helix bundle-cys/porphyrin is dialysed into PBS and concentrated as above.

Provenance & Evidence