Bromoacetyl bromide → HCID 136378435, compound
Reaction equation
C12C=C3N=C(C=C3)C=C3NC(C=C3)=CC3=NC(C=C3)=CC(N1)=CC=2.[NH2:25][C:26]1[CH:27]=[C:28]([C:32]2[C:33]3[NH:37][C:36]([C:38]([C:68]4[CH:73]=[CH:72][CH:71]=[CH:70][CH:69]=4)=[C:39]4[N:67]=[C:42]([C:43]([C:61]5[CH:66]=[CH:65][CH:64]=[CH:63][CH:62]=5)=[C:44]5[NH:60][C:47](=[C:48]([C:54]6[CH:59]=[CH:58][CH:57]=[CH:56][CH:55]=6)[C:49]6[CH:50]=[CH:51][C:52]=2[N:53]=6)[CH:46]=[CH:45]5)[CH:41]=[CH:40]4)=[CH:35][CH:34]=3)[CH:29]=[CH:30][CH:31]=1.[Br:74][CH2:75][C:76](Br)=[O:77]>>[Br:74][CH2:75][C:76]([NH:25][C:26]1[CH:27]=[C:28]([C:32]2[C:33]3[NH:37][C:36]([C:38]([C:68]4[CH:69]=[CH:70][CH:71]=[CH:72][CH:73]=4)=[C:39]4[N:67]=[C:42]([C:43]([C:61]5[CH:62]=[CH:63][CH:64]=[CH:65][CH:66]=5)=[C:44]5[NH:60][C:47](=[C:48]([C:54]6[CH:59]=[CH:58][CH:57]=[CH:56][CH:55]=6)[C:49]6[CH:50]=[CH:51][C:52]=2[N:53]=6)[CH:46]=[CH:45]5)[CH:41]=[CH:40]4)=[CH:35][CH:34]=3)[CH:29]=[CH:30][CH:31]=1)=[O:77]Participants
Procedure
A porphyrin (5-(3-aminophenyl)-10,15,20-triphenylporphyrin is derivatized by reacting with excess bromoacetyl-bromide (dissolved in acetone containing sodium carbonate) to form (5-(3-bromoacetamidophenyl)-10,15,20-triphenylporphyrin. 10 μmol of the derivatized porphyrin and 10 mg of the scFv-4-helix bundle-cys are dissolved separately in 20 ml of dry dimethyl formamide. These two solutions are added over a period of 3 hours to 50 ml dry dimethyl formamide containing 25 mg sodium carbonate. These reactions are performed in the dark, under argon. The final product is scFv-4-helix bundle-cys with optimally conjugated porphyrins (scFv-4-helix bundle-cys/porphyrin). The scFv-4-helix bundle-cys/porphyrin is dialysed into PBS and concentrated as above.
Sources & evidence
- ORD record
- ord-c71a1883b81b4ff59c3bc8cd6263195d
- Source dataset
- uspto-grants-2010_02
- Patent number
- US07655753B2
Reaction notes
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