反应详情

A solution of tert-butyl [3-(cyclopropylmethyl)-2-oxo-1,3-diazepan-1-yl]acetate from Step D (240 mg, 0.85 mmol) in CH2Cl2 (5 mL) and CF3CO2H (2 mL) was stirred at ambient temperature for 3 h. The mixture was concentrated in vacuo and the residue was purified by HPLC using a reversed phase C18 column and eluting with a gradient of H2O:CH3CN:CF3CO2H—90:10:0.1 to 5:95:0.1. Lyophilization provided the title compound. MS: m/z=227 (M+1).

1. concentrationThe mixture was concentrated in vacuo
2. customthe residue was purified by HPLC
3. washeluting with a gradient of H2O