反应详情

温度

0 °C

Compound 50 (200 mg, 0.38 mmol) was dissolved in a mixture of methanol/THF/water, 1:2:1, (20 ml) and cooled on ice-bath. Lithium hydroxide (1.9 ml, 1M) was added slowly. The mixture was stirred for 4 h at 0° C., then neutralized with aqueous acetic acid (20 ml) and extracted with dichloromethane. The organic phase was washed with bicarbonate, water, brine and dried over magnesium sulfate. Purification by chromatography (2% methanol in dichloromethane→4%) gave the title compound as a greyish powder (80%).

1. temperaturecooled on ice-bath
2. extractionextracted with dichloromethane
3. washThe organic phase was washed with bicarbonate, water, brine
4. dry with materialdried over magnesium sulfate
5. customPurification by chromatography (2% methanol in dichloromethane→4%)