Citric Acid → HCID 8029997, compound
Reaction equation
C(O)(=O)CC(CC(O)=O)(C(O)=O)O.O.C(#N)C.[C:18]([O:34][CH2:35][CH3:36])(=[O:33])[CH2:19][C:20]([CH2:27][C:28]([O:30][CH2:31][CH3:32])=[O:29])([C:22]([O:24]CC)=[O:23])[OH:21]>C(#N)C>[C:28]([O:30][CH2:31][CH3:32])(=[O:29])[CH2:27][C:20]([CH2:19][C:18]([O-:34])=[O:33])([C:22]([O-:24])=[O:23])[OH:21].[C:28]([O:30][CH2:31][CH3:32])(=[O:29])[CH2:27][C:20]([CH2:19][C:18]([O:34][CH2:35][CH3:36])=[O:33])([C:22]([O-:24])=[O:23])[OH:21]Participants
Reactant 3
Product 2
Reagents, catalysts & solvents 1
Procedure
Citric acid and its ethyl esters (mono, di and tri) were quantitatively analyzed on a Hewlett-Packard 1090 HPLC using a reversed phase C18 column (Novapak, 3.9 mm×150 mm) held at 40° C. Water/acetonitrile (ACN) mixtures, buffered at pH=1.3, were used as mobile phase (1.0 ml/min) in a gradient mode (0% ACN (t=0) to 60% ACN (t=20 min) to 90% ACN (t=25 min) to 0% ACN (t=28 min)), and species were quantified by UV detection (Hitachi L400H) at a wavelength of 210 nm. Citric acid (CA) and triethyl citrate (TEC) were identified and quantified by comparing HPLC retention time and peak area with their respective calibration standard. Standards for monoethyl citrate (MEC) and diethyl citrate (DEC) could not be obtained commercially. On a mass basis, the response factor values for CA and MEC were found to be the same; therefore MEC and DEC were each assigned the same response factor value as corresponding to TEC. Using these response factor values, the carbon balance for each reaction sample, based on citric acid and its esters, was in the range of ±10%.
Workup
- customheld at 40° C
Sources & evidence
- ORD record
- ord-48a549b136d64e40a7d2f47dadef829c
- Source dataset
- uspto-grants-2010_02
- Patent number
- US07667068B2
Reaction notes
Loading…