Ethanol → HCID 53945635, compound

A solution of 234 mg (0.91 mmol) of 1-(6-deoxy-β-D-glucopyranosyl)-3-methyl-3-nitrosourea in 2 ml of water was admixed with a solution of 401 mg (3.5 mmol) of 2-chloroethylamine hydrochloride and 0.5 ml (3.3 mmol) of triethylamine in 3 ml of aqueous 50% ethanol. The admixture was stirred for 2 hours at ambient temperature, and the reaction mixture was passed through a column (10×160 mm) of Amberlite IRA-400 (OH- form), which was then washed with water. The effluent was concentrated to dryness under a reduced pressure, and the crystalline residue so obtained was subjected to chromatography in a column (8×350 mm) of silica gel (8 g) using chloroformmethanol (5:2 by volume) as eluent. Such fractions of the eluate which gave a single spot at Rf 0.50 were combined together and concentrated to dryness by evaporation of the solvent under a reduced pressure. Recrystallization of the residue from acetone gave the titled compound. Yield 98 mg (37.7%). mp. 130°-132° C. [α]D24 -25.5° (c 0.5, water).

1. washwas then washed with water
2. concentrationThe effluent was concentrated to dryness under a reduced pressure
3. customthe crystalline residue so obtained
4. customSuch fractions of the eluate which gave a single spot at Rf 0.50
5. concentrationconcentrated to dryness by evaporation of the solvent under a reduced pressure
6. customRecrystallization of the residue from acetone

ORD record

ord-daa96d640f474fb68e916ad835f29f9c

Source dataset

uspto-grants-1979_12

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US04180655