Pyridoxal 5'-phosphate → 2-Amino-4-cyanobutanoic acid

HRID 1457397Notes

Reaction equation

HRID 1457397 reaction equation
CC1[N:7]=[CH:6]C(COP(O)(O)=O)=C(C=O)C=1O.C(O[CH2:21][CH2:22][C@@H:23]([C:25]([OH:27])=[O:26])[NH2:24])(=O)C>OP([O-])([O-])=O.[K+].[K+]>[C:6]([CH2:21][CH2:22][CH:23]([NH2:24])[C:25]([OH:27])=[O:26])#[N:7]

Participants

4

Reactant 2

HCID 1051Pyridoxal 5'-phosphateHCID 439389O-Acetyl-L-homoserine

Product 1

HCID 4407702-Amino-4-cyanobutanoic acid

Reagents, catalysts & solvents 1

HCID 24450Potassium Phosphate, DibasicSolvent2×

Procedure

Then, 250 μl 200 mM K2HPO4, 10 μl 10 mM pyridoxal phosphate (PLP), 110 μl 25 mM O-acetyl-L-homoserine (OAHS) dissolved in 100 mM K2HPO4, and γ-cyano-α-aminobutyric acid synthase (GCAs) obtained in Example 5 were added to this trapped H11 CN, and the mixture was subjected to enzymatic reaction at 65° C. for 10 minutes.

Sources & evidence

ORD record
ord-1502bfdbd19043d5a0440fa572d2fae9
Source dataset
uspto-grants-1998_12
Patent number
US05847193

Reaction notes

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