反应详情

Then, 250 μl 200 mM K2HPO4, 10 μl 10 mM pyridoxal phosphate (PLP), 110 μl 25 mM O-acetyl-L-homoserine (OAHS) dissolved in 100 mM K2HPO4, and γ-cyano-α-aminobutyric acid synthase (GCAs) obtained in Example 5 were added to this trapped H11 CN, and the mixture was subjected to enzymatic reaction at 65° C. for 10 minutes.