反应详情

To a solution of (1-tert-butoxycarbonylbut-3-enylamino)-cyclohexanecarboxylic acid (0.5 g, 1.29 mmol) in dioxane (5 mL) and water (5 mL) was added a 2.5 weight % solution of osmium tetroxide in tert-butanol (0.05 mL). After 20 minutes, sodium periodate (0.88 g, 4.13 mmol) was added in small portions over another period of 20 minutes. The mixture was stirred at room temperature for 6 hours and was quenched by addition of a saturated aqueous solution of sodium bicarbonate. The mixture was extracted with ethyl acetate and the extract was washed with brine, dried over magnesium sulfate, and concentrated under vacuum to give an oil. The oil was dissolved in methanol (12 mL) and sodium borohydride (137 mg, 3.61 mmol) was added. The mixture was stirred overnight. A 1M aqueous solution of hydrochloric acid was added and, after stirring for 10 minutes, the solvent was removed under vacuum. The residue was partitioned between a saturated aqueous solution of sodium bicarbonate and ethyl acetate. The organic layer was washed with brine dried over magnesium sulfate, and concentrated under vacuum to give an oil. The oil was chromatographed on silica gel eluting with 10% ethyl acetate in methylene chloride to provide 1-(1-tert-butoxycarbonyl-3-hydroxy-propylamino)cyclohexanecarboxylic acid benzyl ester as an oil, 469 mg (66%).

1. customwas quenched by addition of a saturated aqueous solution of sodium bicarbonate
2. extractionThe mixture was extracted with ethyl acetate
3. washthe extract was washed with brine
4. dry with materialdried over magnesium sulfate
5. concentrationconcentrated under vacuum
6. customto give an oil
7. stirringThe mixture was stirred overnight
8. stirringafter stirring for 10 minutes
9. customthe solvent was removed under vacuum
10. customThe residue was partitioned between a saturated aqueous solution of sodium bicarbonate and ethyl acetate
11. washThe organic layer was washed with brine
12. dry with materialdried over magnesium sulfate
13. concentrationconcentrated under vacuum
14. customto give an oil
15. customThe oil was chromatographed on silica gel eluting with 10% ethyl acetate in methylene chloride