Citric Acid → N-(4-azido-2-nitrophenyl)-N'-(N-((2-hydroxyethyldithio)-2-ethylamidosuccinimido)-3-aminopropyl)-N'-methyl-1,3-propanediamine citrate salt

Participants

Reaction Steps

The reaction was carried out in the dark. A flask charged with a solution of 1.15 (2.12 mmole) of N-(4-azido-2-nitrophenyl)-N'-(N-((2-hydroxyethyldithio)-2-ethylamidosuccinimido)-3-aminopropyl)-N'-methyl-1,3-propanediamine in a minimal amount of methanol was treated with a solution of 407.1 mg (2.12 mmole) citric acid dissolved in a minimal amount of methanol at room temperature. After stirring for 5 min the volatiles where removed in vacuo to give 1.4027 g of the desired product as a red solid.

Provenance & Evidence