Hydrochloric Acid → L-Lysine

Participants

Reaction Steps

To 200 mM of sodium phosphate buffer solution (pH7.0) containing 200 mM of L-lysine monohydrochloride and 0.15 mM of pyridoxal phosphate (manufactured by Hiroshima Wako Ltd.), a bacterial cell suspension or a treated bacterial cell suspension was added, thereby preparing a total mixture of 0.2 mL, and the mixture was allowed to react at 37° C. for 6 minutes. To the reaction liquid, 1 mL of hydrochloric acid (0.2M) was added, thereby terminating the reaction. The reaction terminated liquid was diluted with water appropriately, and the produced 1,5-pentamethylenediamine was determined by HPLC.

Provenance & Evidence