Hydrochloric Acid → L-Lysine

HRID 1478954Notes

Reaction equation

HRID 1478954 reaction equation
P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+].Cl.[NH2:10][C@H:11]([C:17]([OH:19])=[O:18])[CH2:12][CH2:13][CH2:14][CH2:15][NH2:16].CC1N=CC(COP(O)(O)=O)=C(C=O)C=1O.Cl>>[NH2:10][C@H:11]([C:17]([OH:19])=[O:18])[CH2:12][CH2:13][CH2:14][CH2:15][NH2:16]

Participants

5

Reactant 4

HCID 313Hydrochloric AcidHCID 1051Pyridoxal 5'-phosphateHCID 24243Trisodium PhosphateHCID 69568L-Lysine monohydrochloride

Product 1

HCID 5962L-Lysine

Procedure

To 200 mM of sodium phosphate buffer solution (pH7.0) containing 200 mM of L-lysine monohydrochloride and 0.15 mM of pyridoxal phosphate (manufactured by Hiroshima Wako Ltd.), a bacterial cell suspension or a treated bacterial cell suspension was added, thereby preparing a total mixture of 0.2 mL, and the mixture was allowed to react at 37° C. for 6 minutes. To the reaction liquid, 1 mL of hydrochloric acid (0.2M) was added, thereby terminating the reaction. The reaction terminated liquid was diluted with water appropriately, and the produced 1,5-pentamethylenediamine was determined by HPLC.

Workup

  1. additiona bacterial cell suspension or a treated bacterial cell suspension was added
  2. custompreparing
  3. additiona total mixture of 0.2 mL
  4. customto react at 37° C. for 6 minutes
  5. additionwas added
  6. customterminating
  7. customthe reaction
  8. customThe reaction terminated liquid
  9. additionwas diluted with water appropriately

Sources & evidence

ORD record
ord-d246749525e44fba9117603ea49becad
Source dataset
uspto-grants-2016_08
Patent number
US09404132B2

Reaction notes

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