L-Glutamine → L-Aspartic Acid

Participants

Reaction Conditions

Reaction Steps

10 mM L-glutamine or 10 mM L-asparagine in 0.1 M potassium phosphate pH 7.0, respectively, were preheated to 37° C. The resulting assay was started by addition of 50 μl native or 10 μl recombinant enzyme in a total reaction volume of 150 μL and stopped after 10-20 min by addition of 20 μL 3% TCA or by heating at 95° C. for 10 min. A control experiment was carried out without amino acids. Formation of product was followed using HPLC. One unit of enzyme activity was calculated as the amount of enzyme required to produce 1 μM glutamic acid or aspartic acid respectively, at 37° C. per minute.

Provenance & Evidence