Phosphoric Acid → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 1488378Notes

Reaction equation

HRID 1488378 reaction equation
P(=O)(O)(O)O.[Cl-].[Na+].[CH2:8]([OH:30])[C@H:9]1[O:14][C@H:13]([O:15][C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H:12]([OH:27])[C@@H:11]([OH:28])[C@@H:10]1[OH:29]>O>[O:15]=[CH:13][C@@H:12]([C@H:11]([C@@H:10]([C@@H:9]([CH2:8][OH:30])[OH:14])[OH:29])[OH:28])[OH:27]

Participants

5

Reactant 3

HCID 1004Phosphoric AcidHCID 5234Sodium ChlorideHCID 6255beta-Maltose

Product 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

To 1 ml of a buffer solution (pH 6.5) containing 20 mM phosphoric acid and 6.7 mM sodium chloride, 300 mg of maltose (Wako Pure Chemical Industries Ltd. product) were dissolved, and then, 10 mg of α-amylase (saccharification type, Seikakagaku Corporation product by using Bacillus subtilis) and 1 mg of ε-poly-L-lysine hydrochloride were added and dissolved. To this solution, 103 cells of Bacillus stearothermophilus were inoculated and then, were allowed to react at 60° C. for 24 hrs. After the reaction was completed, the reaction solution was heated in a boiled water to stop the enzyme reaction, and filtered through a 0.45 μm membrane filter. The quantity of glucose in the filtrate was measured by a high speed liquid chromatographic method. The quantity of glucose thus obtained was 260 mg.

Workup

  1. dissolutionwere dissolved
  2. dissolutiondissolved
  3. customto react at 60° C. for 24 hrs
  4. customthe enzyme reaction
  5. filtrationfiltered through a 0.45 μm membrane
  6. filtrationfilter

Sources & evidence

ORD record
ord-bc5564e827664d5f9972066b10038d5a
Source dataset
uspto-grants-1997_10
Patent number
US05674726

Reaction notes

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