Phosphoric Acid → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 1488379Notes

Reaction equation

HRID 1488379 reaction equation
P(=O)(O)(O)O.[Cl-].[Na+].[CH2:8]([OH:30])[C@H:9]1[O:14][C@H:13]([O:15][C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H:12]([OH:27])[C@@H:11]([OH:28])[C@@H:10]1[OH:29]>O>[O:15]=[CH:13][C@@H:12]([C@H:11]([C@@H:10]([C@@H:9]([CH2:8][OH:30])[OH:14])[OH:29])[OH:28])[OH:27]

Participants

5

Reactant 3

HCID 1004Phosphoric AcidHCID 5234Sodium ChlorideHCID 6255beta-Maltose

Product 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

To 1 ml of a buffer solution containing 20 mM phosphoric acid and 6.7 mM sodium chloride, 300 mg of maltose (Wako Pure Chemical Industries Ltd. product) were dissolved, and then, 10 mg of α-amylase (saccharification type, Seikakagaku Corporation product by using Bacillus subtilis) and 1 mg of ε-poly-L-lysine hydrochlorate were added and dissolved. The resulting solution was allowed to react at 60° C. for 24 hrs. After the reaction was completed, the reaction solution was heated in a boiled water to stop the enzyme reaction, and filtered through a 0.45 μm membrane filter. The quantity of glucose in the filtrate was measured by a high speed liquid chromatographic method. The quantity of glucose thus obtained was 265 MG.

Workup

  1. dissolutionwere dissolved
  2. dissolutiondissolved
  3. customto react at 60° C. for 24 hrs
  4. customthe enzyme reaction
  5. filtrationfiltered through a 0.45 μm membrane
  6. filtrationfilter

Sources & evidence

ORD record
ord-401a2d49dfb04a45b724037bcab6dbe0
Source dataset
uspto-grants-1997_10
Patent number
US05674726

Reaction notes

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