Phosphoric Acid → aldehydo-D-Xylose

Participants

Reaction Steps

To 1 ml of a buffer solution (pH 6.8) containing 20 mM phosphoric acid and 6.7 mM sodium chloride, 10 mg of glucose was dissolved, and then, 10 mg of xylose isomerase prepared by using Bacillus coagulans) was added and dissolved. The spores of Bacillus cereus (equivalent to 103 colony-forming unites) were inoculated to the solution and then allowed to react at30° C. for 2 days. After the reaction was completed, the reaction solution was heated in a boiled water to stop the enzyme reaction. The solution was left at 30° C. for 24 hrs. and then filtered through a 0.45 μm membrane filter. The quantities of fructose and glucose in the filtrate were measured by a high speed liquid chromatographic method (column: Showa denko K.K. product, #SC1011). Both fructose and glucose were not detected.

Provenance & Evidence