Phosphoric Acid → aldehydo-D-Xylose

HRID 1488381Notes

Reaction equation

HRID 1488381 reaction equation
P(=O)(O)(O)O.[Cl-].[Na+].[O:8]=[CH:9][C@@H:10]([C@H:12]([C@@H:14]([C@@H:16](CO)[OH:17])[OH:15])[OH:13])[OH:11]>O>[O:8]=[CH:9][C@@H:10]([C@H:12]([C@@H:14]([CH2:16][OH:17])[OH:15])[OH:13])[OH:11]

Participants

5

Reactant 3

HCID 1004Phosphoric AcidHCID 5234Sodium ChlorideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 644160aldehydo-D-XyloseYield 120%

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

To 1 ml of a buffer solution (pH 6.8) containing 20 mM phosphoric acid and 6.7 mM sodium chloride, 10 mg of glucose was dissolved, and then, 10 mg of xylose isomerase prepared by using Bacillus coagulans) was added and dissolved. The spores of Bacillus cereus (equivalent to 103 colony-forming unites) were inoculated to the solution and then allowed to react at30° C. for 2 days. After the reaction was completed, the reaction solution was heated in a boiled water to stop the enzyme reaction. The solution was left at 30° C. for 24 hrs. and then filtered through a 0.45 μm membrane filter. The quantities of fructose and glucose in the filtrate were measured by a high speed liquid chromatographic method (column: Showa denko K.K. product, #SC1011). Both fructose and glucose were not detected.

Workup

  1. dissolutionwas dissolved
  2. additionwas added
  3. dissolutiondissolved
  4. customto react at30° C
  5. customthe enzyme reaction
  6. waitThe solution was left at 30° C. for 24 hrs
  7. filtrationand then filtered through a 0.45 μm membrane
  8. filtrationfilter

Sources & evidence

ORD record
ord-8dfe2de33405455381c7440dafd05760
Source dataset
uspto-grants-1997_10
Patent number
US05674726

Reaction notes

Loading…