Ammonia → L-Aspartic Acid

HRID 1488922Notes

Reaction equation

HRID 1488922 reaction equation
C(O)(=O)/C=C\C(O)=O.N.N#N.S(=O)(=O)(O)O.[NH2:17][C@H:18]([C:23]([O-:25])=[O:24])[CH2:19][C:20]([O-:22])=[O:21].[NH4+].[NH4+]>O>[NH2:17][C@H:18]([C:23]([OH:25])=[O:24])[CH2:19][C:20]([OH:22])=[O:21]

Participants

7

Reactant 5

HCID 222AmmoniaHCID 947Nitrogen3×HCID 1118Sulfuric AcidHCID 444266Maleic AcidHCID 86590683Ammonium Aspartate

Product 1

HCID 5960L-Aspartic Acid

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

A reaction solution containing maleic acid (116 g) and ammonia (153 ml) (having a total volume of 1,000 ml by adding water), which had been previously subjected to N2 substitution by carrying out agitation while blowing N2 gas in it for 30 minutes, was transferred into a jar fermenter having a volume of 3 L. The recovered microbial cells of the both species (IFO 12669 strain: 20 g, AB-41 strain: 120 g) were added thereto, followed by agitation while supplying N2 at a velocity of 0.02 vvm to carry out a reaction at 30° C. for 36 hours with sealing. After completion of the reaction, a supernatant was recovered by centrifugation. The obtained supernatant of the reaction solution was subjected to thin layer chromatography (developing solvent: n-butanol: acetic acid: water=4:1:1 (volume ratio), coloring agent: ninhydrin reagent). As a result, it was confirmed that 128 g/L of L-aspartic acid was produced. Sulfuric acid was added to this ammonium aspartate solution to adjust pH to 3. Thus aspartic acid was precipitated, washed with water, and then dried to obtain crystals of aspartic acid. The obtained crystals had an amount of 125 g.

Workup

  1. customfor 30 minutes
  2. additionThe recovered microbial cells of the both species (IFO 12669 strain: 20 g, AB-41 strain: 120 g) were added
  3. customa reaction at 30° C. for 36 hours with sealing
  4. customAfter completion of the reaction
  5. customa supernatant was recovered by centrifugation
  6. customwas produced
  7. customThus aspartic acid was precipitated
  8. washwashed with water
  9. customdried

Sources & evidence

ORD record
ord-48b316899f2749b0a86ea842fea321df
Source dataset
uspto-grants-1997_10
Patent number
US05677156

Reaction notes

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