反应详情

The resulting compound was deprotected, by treatment with 1M sodium hydroxide (500 microliter) for 30 minutes at room temperature in the dark. The reaction mixture was taken to pH 3 with 2M hydrochloric acid (200 microliter) and then 1M citric acid (1.5 ml). Volatiles were removed in vacuo, the residue taken up in methanol, and inorganics filtered to leave a clear yellow solution. This was subjected to TLC (eluent dichloromethane-methanol-acetic acid 75:25:1) to give the product as one major band Rf 0.10, which was eluted with methanol and stored at -20° C. in the dark.

1. customVolatiles were removed in vacuo
2. filtrationinorganics filtered
3. customto leave a clear yellow solution
4. customto give the product as one major band Rf 0.10, which
5. washwas eluted with methanol
6. customstored at -20° C. in the dark